Residual Endotoxin Activates Dendritic Cells

Over the past few years this newsletter has highlighted several publications indicating the importance of the detection and removal of even sub-clinical levels of endotoxin (e.g., 2013 PLoS One, 2014 JBC). A recent publication from the University of Salzburg further emphasizes this.

Most commercially available recombinant proteins guarantee an endotoxin level less than 1 EU/microgram. The study, published in PLoS One, found that some commercial preps contained more endotoxin than indicated. Additionally, using an array of cytokine assays (IL-6, IL-8, TNFα), NF-κB activation, IL-1β mRNA levels and surface marker expression (CD40, CD80, CD83, CD86) they found that accurately labeled proteins could activate dendritic cells with residual endotoxin levels as low as 0.2 EU/microgram.

BioDtech offers two solutions to the problem of residual endotoxin contamination. First, our EndoBind-R™ technology has been shown to remove even trace amounts of endotoxin. Second, our EndoPrep™ kits can remove interfering proteins to elucidate masked endotoxin not detectable with untreated samples.

Residual Endotoxin Contaminations in Recombinant Proteins are Sufficient to Activate Human CD1c+ Dendritic Cells

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